Abstract
Summary Poly(U) isolated from polio [32P]RF has the base composition (U65, C2)Gp. Labeling of the poly(U) in RF with NaIO4/[3H]NaBH4 failed, even if the RF was pretreated with phosphatase. Labeling of poly(U) in RF with [γ-32P]ATP and polynucleotide kinase occurs but only after treatment of the RF with either snake venom exonuclease or polynucleotide phosphorylase. The data suggest that poly(U) is the 5′-end of polio minus RNA.
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More From: Biochemical and Biophysical Research Communications
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