Abstract
An acid fraction from polyacrylamide gel isoelectric focusing contained only DNA polymerase template activity for (rC) n-(dG) 12–18 with little or no activity with (rA) n-(dT) 12–18. A factor which stimulated (rC) n-(dG) 12–18 activity was also detected in low molecular weight fractions from G-100 Sephadex chromatography of the purified enzyme. Since (rC) n-(dG) 12–18 acitivty is thought to be descriptive of viral DNA polymerase, this stimulation is proposed to be significant for the regulation and specificity of viral DNA synthesis.
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