Poly(MPC-co-BMA) Coating Reduces the Adhesion of Candida albicans to Poly(Methyl Methacrylate) Surfaces

Abstract

Purpose: The aim of the present study was to develop a new and simple method using PMB to prevent plaque colonization on denture surfaces.Methods: PMB polymerized with MPC polymer and BMA was prepared as a coating material. Water-insoluble PMB composed of 30 mol% MPC units (PMB30), water-soluble PMB composed of 80 mol% MPC units (PMB80) and clear PMMA disks were prepared. The PMMA disks were dip-coated in 0.5 wt% PMB30 solution or 0.5 wt% PMB80 solution. To examine the PMB coatings' durability, the PMB-coated disks were immersed in distilled water. The PMB elution ratio was measured using a phosphorus analysis. To evaluate the effect of the PMB coating on Candida albicans (C. albicance) adhesion, PMB-coated and uncoated disks were cultured in a solution containing C. albicans for 48 hours. Half of the disks in each group were not washed, and the remaining disks were washed with distilled water to examine the effect of washing. All the PMMA disks were observed and photographed, and the resulting images were analyzed using imaging analysis software.Results: In the first 48 hours, approximate 14 % of the PMB30-coating and 12 % of the PMB80-coating were eluted into the distilled water. The adhesion of C. albicans to the PMB30-coated or the PMB80-coated PMMA disks was significantly reduced in both the washed and unwashed groups, compared with the uncoated control disks.Conclusion: PMB coating prevents the adhesion of C. albicans to PMMA.