Abstract
Poly-L-Lactic acid (PLLA) blended with chitosan and collagen was used to fabricate a conduit for blood vessel engineering through an electrospinning process. Various concentrations of chitosan were used in the blend in order to study its effect on the morphology, chemical bond, tensile strength, burst pressure, hemocompatibility, and cell viability (cytotoxicity) of the tube.In vitro assessments indicated that addition of chitosan-collagen could improve cell viability and hemocompatibility. Best results were demonstrated by the conduit with 10% PLLA, 0.5% chitosan, and 1% collagen. Tensile strength reached 2.13 MPa and burst pressure reached 2593 mmHg, both values that are within the range value of native blood vessel. A hemolysis percentage of 1.04% and a cell viability of 86.2% were obtained, meeting the standards of high hemocompatibility and low cytotoxicity for vascular graft material. The results are promising for further development toward vascular graft application.
Highlights
Cardiovascular diseases (CVDs) are the leading cause of non-communicable disease death
The three peaks were found in each concentration variation of sample B (0.5%:1%:10%; chi:col:Poly-L-Lactic acid (PLLA)) and sample C (0.6%:1%:10%; chi:col:PLLA), the most prominent peaks were found in sample A, and belonged to the ester group, indicating that the only polymer in sample A was PLLA
Sample B (0.5%:1%:10%; chi:col:PLLA) and sample C (0.6%:1%:10%; chi:col:PLLA) had hemolytic percentages of 1.04% and 3.14%, respectively, passing the criteria of hemolytic percentage allowed in vascular prostheses application issued in ASTM-F756-00
Summary
Cardiovascular diseases (CVDs) are the leading cause of non-communicable disease death. In 2014, the WHO reported that CVDs killed 17.5 million people worldwide, 46% of total non-communicable deaths. Atherosclerosis is commonly treated with vascular graft bypass [2]. Material for vascular grafts must be biocompatible, non-thrombogenic, hemocompatible, and have suitable mechanical and physical characteristics [2]. Synthetic vascular grafts have gained popularity among medical practitioners for their ease of fabrication and handling. Thrombogenesis happens when endothelial tissue hyperplasia occurs as a result of mechanical characteristic mismatches between the graft and native vessel [3]. Most commercial synthetic grafts are based on non-degradable polymers (e.g., Dacron, ePTFE), which cannot be decomposed by the human body [4]. The graft will maintain its original form, while the surrounding tissue
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
