Poly-L-arginine Coated Silver Nanoprisms and Their Anti-Bacterial Properties.

Fouzia Tanvir,Atif Yaqub,Shazia Tanvir,William A Anderson

doi:10.3390/nano7100296

Abstract

The aim of this study was to test the effect of two different morphologies of silver nanoparticles, spheres, and prisms, on their antibacterial properties when coated with poly-L-arginine (poly-Arg) to enhance the interactions with cells. Silver nanoparticle solutions were characterized by UV–visible spectroscopy, transmission electron microscopy, dynamic light scattering, zeta potential, as well as antimicrobial tests. These ultimately showed that a prismatic morphology exhibited stronger antimicrobial effects against Escherichia coli, Pseudomonas aeruginosa and Salmonella enterica. The minimum bactericidal concentration was found to be 0.65 μg/mL in the case of a prismatic AgNP-poly-Arg-PVP (silver nanoparticle-poly-L-arginine-polyvinylpyrrolidone) nanocomposite. The anticancer cell activity of the silver nanoparticles was also studied, where the maximum effect against a HeLa cell line was 80% mortality with a prismatic AgNP-poly-Arg-PVP nanocomposite at a concentration of 11 μg/mL. The antimicrobial activity of these silver nanocomposites demonstrates the potential of such coated silver nanoparticles in the area of nano-medicine.

Highlights

Silver has been known to have strong inhibitory or bactericidal effects, with a broad spectrum
Spherical AgNPs were prepared using AgNO3, NaBH4, and trisodium citrate [34], but using the same reaction mixture nanoprism, AgNPs were synthesized by the addition of H2O2
Size and shape were characterized by UV-vis spectroscopy, TEM and EDX

Summary

Introduction

Silver has been known to have strong inhibitory or bactericidal effects, with a broad spectrum. Silver nanoparticles (AgNPs) are utilized in an increasing number of medical and other products including cosmetics, textiles, electronics, paints, and water disinfectants due to their antibacterial properties [1]. Silver nanoparticles have been studied for analytical measurements making use of the shift in the wavelength of these plasmonic nanoparticles when they interact with molecules [2,3]. It has been reported that stable nanosilver does have a much lower minimal inhibitory concentration than its dissolved ionic counterpart [5], and silver ions in solution are powerful antimicrobials, but they are sequestered by chloride, phosphate, proteins, and other cellular components [6]

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