Poly(hydroxyethyl methacrylate) membranes: as a hydrogel support for use in immobilized metal affinity chromatography

Abstract

AbstractLysozyme adsorption onto a dye ligand (Procion Red HE‐3B) immobilized and Cu(II) incorporated poly(2‐hydroxyethylmethacrylate) (pHEMA) membrane, were investigated. The membranes were prepared by UV initiated photopolymerization of HEMA in the presence of an initiator (α‐α′‐azoisobutyronitrile; AIBN). The amount of immobilized dye on the membrane was 112.2 μmol g−1. Lysozyme adsorption on to these membranes from aqueous solutions containing different amounts of lysozyme at different pH was investigated in batch system. Lysozyme adsorption capacity of the dye‐ligand immobilized membrane was 45.6 mg g−1. Lysozyme adsorption capacity of the Cu(II) incorporated membranes (112.3 mg g−1) was greater than that of the Procion Red HE‐3B immobilized membranes. The non‐specific adsorption of lysozyme on the pHEMA membranes was 0.14 mg g−1. More than 97% of the adsorbed lysozyme were desorbed in 60 in the desorption medium containing 1.0 M KSCN at pH 8.0.