Poly herbal formulation with anti-elastase and anti-oxidant properties for skin anti-aging

Sahabudeen Sheik Mohideen,Shinomol George,Vignesh Sundararajan,Deepika Deeptirekha Sarangi,Induja Kalyana Sundaram

doi:10.1186/s12906-018-2097-9

Sahabudeen Sheik Mohideen, Shinomol George + Show 3 more

Open Access

https://doi.org/10.1186/s12906-018-2097-9

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Abstract

BackgroundSkin forms an important part of human innate immune system. Wrinkles, thinning and roughening of skin are some of the symptoms that affect the skin as it ages. Reactive oxygen species induced oxidative stress plays a major role in skin aging by modulating the elastase enzyme level in the skin. Extrinsic factors that affect skin aging such as UV radiation can also cause malignant melanoma. Here we selected four medicinal plant materials, namely, leaves of Nyctanthes arbor-tristis, unripe and ripe Aegle marmelos fruit pulp and the terminal meristem of Musa paradisiaca flower and investigated their anti-aging properties and cytotoxicity in vitro individually as well as in a poly herbal formulation containing the four plant extracts in different ratios.MethodsThe phytochemical contents of the plant extracts were investigated for radical scavenging activity and total reducing power. Based upon its anti-oxidant properties, a poly herbal formulation containing leaves of Nyctanthes arbor-tristis, unripe and ripe fruit pulp of Aegle marmelos, and the terminal meristem of Musa paradisiaca flower in the ratio 6:2:1:1 (Poly Herbal Formulation 1) and 1:1:1:1 (Poly Herbal Formulation 2), respectively were formulated.ResultIt has been observed that the Poly Herbal Formulation 1 was more potent than Poly Herbal Formulation 2 due to better anti-oxidant and anti-elastase activities in NIH3T3 fibroblast cells. In addition Poly Herbal formulation 1 also had better anti-cancer activity in human malignant melanoma cells.ConclusionBased on these results these beneficial plant extracts were identified for its potential application as an anti-aging agent in skin creams as well as an anti-proliferation compound against cancer cells.

Highlights

Skin forms an important part of human innate immune system
Chemicals and reagents 2,2-Diphenyl-1-picrylhydrazyl (DPPH), methanol, ascorbic acid, sodium nitroprusside, curcumin, sodium chloride, Griess reagent, porcine pancreatic elastase (EC.3.4.21.36), N-succinyl-Ala-Ala-Ala-p-nitroanilide, tris base, hydrochloric acid, copper sulphate, ferric chloride, trichloroacetic acid, potassium ferricyanide, dimethyl sulfoxide (DMSO), 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT), Dulbecco’s Modified Eagle Medium (DMEM), fetal bovine serum (FBS), Mayer’s reagent, lead acetate, chloroform, acetic acid, and sulphuric acid were purchased from Sigma-Aldrich (Bangalore, India)
Preliminary qualitative phytochemical analysis of plant extracts The methanolic extracts of unripe fruit pulp of Aegle marmelos, ripe fruit pulp of Aegle marmelos, leaves of Nyctanthes arbor-tristis, and the terminal meristem of Musa paradisiaca flower were screened for the presence of various bioactive phytochemical compounds

Summary

Introduction

Skin forms an important part of human innate immune system. Wrinkles, thinning and roughening of skin are some of the symptoms that affect the skin as it ages. Extrinsic factors that affect skin aging such as UV radiation can cause malignant melanoma. Skin aging is mainly characterized by wrinkle formation, uneven pigmentation, darkening, thinning, sagging, and roughening of skin [1, 2].This could be caused either by intrinsic or extrinsic mechanisms [3,4,5]. Phytochemicals are important constituents of human food sources and investigation of the anti-oxidant, anti-elastase and antiproliferative properties of polyphenolic compounds present in commonly consumed fruits such as Aegle marmelos (common name – bael) and Musa paradisiaca (common name – banana) would be a more effective approach to treat age-related diseases and skin cancer [18, 19]

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