Abstract
Poly (C)-binding protein 1 (PCBP1), which acts as an RNA binding protein, has multiple functions and regulates gene expression by binding to polycytosine, poly (C). The aim of the present study was to investigate the novel function of PCBP1 in the nervous system microenvironmen. The overexpression of PCBP1 has a critical effect on the proliferation and anti-apoptosis of neuroblastoma and glial cells in the direct model, whereas the overexpression of PCBP1 in neuroblastoma or glial cells cannot affect the proliferation or apoptosis of neuroblastoma or glial cells through substances secreted extracellularly. Fur-thermore, through direct or indirect actions, PCBP1 suppressed the expression of nuclear factor kappa B (NF-κB) and the inflammatory cytokine interleukin (IL)-6 and increased the levels of the anti-inflammatory cytokine IL-10; addi-tionally, PCBP1 changed the expression of functional proteins, such as heat shock protein 70 (HSP70), glial cell line-derived neurotrophic factor (GDNF) and brain-derived neurotrophic factor (BDNF). The results suggest that PCBP1 can regulate the nervous system in proliferation, apoptosis, inflammatory re-sponse and expression of relevant functional proteins, and it could provide novel targets for gene treating human neurological disorders.
Highlights
(C)-binding protein 1 (PCBP1), known as αCP1 or heterogeneous nuclear ribonuclear proteins (hnRNPs) E1, is a bridge protein with multiple biological functions in mammalian cells [1]
This study shows that Poly (C)-binding protein 1 (PCBP1) is closely related to the proliferation, anti-apoptosis, anti-inflammatory and neurotrophic factor secretion of neuroblastoma cells and glial cells, suggesting that PCBP1 plays a significant role in the nervous system microenvironment
After transfection for 48 h, real-time qPCR and western blot revealed that the expression levels of PCBP1 in SH-SY5Y, BV-2 and human astrocyte cell line (HA) cells were significantly increased by pEGFP/N1-PCBP1 when compared to pEGFP/N1 (Figure 2)
Summary
(C)-binding protein 1 (PCBP1), known as αCP1 or hnRNP E1, is a bridge protein with multiple biological functions in mammalian cells [1]. PCBP1 regulates gene expression through the KH structural domain interaction with single- and double-stranded DNA [2] [3]. PCBP1 has been implicated in multiple functions, such as DNA replication, transcriptional regulation, posttranslational modifications and mRNA translation and stabilization [4] [5] [6]. PCBP1 functions as a negative regulator of vesicular stomatitis virus (VSV) to inhibit the expression of virus genes [17]
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