Poly (Bromocresol purple) incorporated pencil graphite electrode for concurrent determination of serotonin and levodopa in presence of L-Tryptophan: A voltammetric study

Abstract

In the present investigation, the new electrochemical sensor was established based on the electropolymerisation of Bromocresol purple on pencil graphite electrode for the sensitive detection of serotonin(5-HT) and Levodopa(LD) in assistance with L- Tryptophan(TRP). The morphological characterisation of developed working electrode was done by Scanning Electron Microscope(SEM). The modified sensor presents admirable electrocatalytic performance towards specific and selective examination of 5-HT, LD and TRP at 0.2 M Phosphate buffer solution of physiological pH having the speed rate of 50 mV/s using cyclic voltammetric(CV) and differential pulse voltammetric(DPV) methods. The distinct experimental conditions like impact of supporting electrolyte, dissimilar concentration and varied sweep rates were optimised to accomplish a better peak current. The designed sensor effectually lowers the detection limits of 5-HT (0.49 μM) and LD (2.3 μM) and it is easy to fabricate, disposable, highly stable and applicable to practical analysis of bioactive molecules.