Abstract
When rat or hen liver nuclei were incubated in the presence of N-methyl-N-nitrosourea (MNU), there was a marked stimulation of the [3H] dTMP incorporation into acid-insoluble fraction. When the incubation was carried out in the presence of MNU plus NAD, the effects of MNU on the DNA synthesis were nil. Furthermore, the incubation of heat-treated nuclei with either partially purified Ca2+, Mg2+ - dependent endonuclease from rat liver or Mg2+ - dependent endonuclease from hen liver (sensitive to poly (ADP-ribose) formation), resulted in MNU-induced stimulation of the DNA synthesis. We propose that the “poly (ADP-ribose)-sensitive endonuclease” may participate in the DNA metabolism in cells exposed to DNA damaging agents.
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