Abstract
Background The spatial distribution of inflammatory and DNA reparation markers in the kidney tissue in diabetes is poorly understood. Aim The present study investigated the role of endothelial and inducible nitric oxide synthase (eNOS and iNOS), Poly ADP ribose polymerase (PARP) xanthine oxidase (XO) in the pathogenesis of streptozotocin-induced diabetic changes in the kidney tissue. Methods Diabetes mellitus was induced in rats by a single injection of streptozotocin (STZ) at a dose of 50 mg/kg. The XO, PARP, eNOS and iNOS protein expression in the kidney was studied by immunohistochemistry. Results Obtained results showed that STZ administration incresed the numbers of PARP and XO-positive cells in kidney tissue compared to control group (respectively, 42 ± 8 vs 10 ± 2, p = 0.01; and 27 ± 7 vs 8 ± 2 cells/mm 2 , p = 0.002). At contrast, the eNOS expression was also observed in medullary collecting tubules, and it was decreased in STZ-treated rats. Conclusion The present study suggests that increased PARP, XO in proximal tubules and decreased eNOS expression in medullary collection tubules, with concomitant increased eNOS and iNOS expression in the microvasculature plays a pivotal role in the kidney damage in diabetes mellitus. Supported by LCS grant Number 278/2012.
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