Abstract
AbstractDirect delivery of exogenous non‐coding nucleic acids into living cells has attracted intense interest in biological applications. However, the cell entry efficiency and target capture ability remain to be improved. Herein, we report a method for compartmenting the nucleic acids on the surface of poly‐adenine‐based spherical nucleic acids (polyA‐SNAs) for efficient capture of oncogenic microRNAs (miRNAs) in living cells. We find that polyA‐SNAs exhibit high cell entry efficiency, which is insensitive to the configuration of the anti‐miRNA sequences. By programming the length of polyAs, we precisely engineered the spatial configuration of the anti‐miRNA sequences in polyA‐SNAs. Compartmentalized polyA‐SNAs bind to miRNAs with improved capture ability as compared to densely compacted SNAs. We further demonstrate that polyA‐SNAs serve as high‐efficacy miRNA sponges for capturing oncogenic miRNAs both in living cells and in mice. The efficient inhibition of miRNAs results in significant suppression of tumor growth.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
