Poly (3‐(3‐Pyridyl) Acrylic Acid) Modified Glassy Carbon Electrode for Simultaneous Determination of Dopamine, Ascorbic Acid and Uric Acid

Abstract

Trans-3-(3-pyridyl) acrylic acid (PAA) was deposited on glassy carbon electrode (GCE) by electropolymerization in pH 7.0 phosphate buffer solution (PBS). The poly (3-(3-pyridyl) acrylic acid) (PPAA) film modified glassy carbon electrode shows an excellent electrochemical response for dopamine (DA), ascorbic acid (AA) and uric acid (UA). The cyclic voltammetry oxidation peaks for DA and AA, DA and UA, AA and UA are separated by 150 mV, 130 mV and 280 mV, respectively. This permits the simultaneous determination of AA, DA and UA. The interference of AA with the determination of DA could be eliminated because of the electrostatic interaction between DA cations and the negatively charged PPAA film at pH 7.0. The anodic peak currents of DA, AA and UA increase linearly with concentration in the range of 1-40 micromol L(-1), 10-400 micromol L(-1) and 1.6-80 micromol L(-1), respectively, with a correlation coefficient (r) always higher than 0.998. The detection limit is 0.06 micromol L(-1), 0.8 micromol L(-1) and 1.1 micromol L(-1) for DA, AA and UA, respectively.