Abstract
In this study, 50 bacterial strains were screened for polyhydroxyalkanoate (PHA) production. The isolated bacterial strain Bacillus flexus ME-77 was found to be the most potent PHA producer. The extracted PHA was analyzed using NMR techniques, which showed that it is of the polyhydroxybutyrate (PHB) type. The highest PHB production (4.5 g/L) was achieved using 30 g/L sugarcane molasses (SCM) at pH 7.5 and applying 1, 0.2 and 0.03 g/L of phosphate, MgSO4 7H2O and CaCl2, respectively, at 27 °C incubation temperature and 200 rpm agitation speed. The bacterial strain Bacillus flexus ME-77 with its potential could be further explored for cost-effective production of PHAs using low-cost agro-industrial food wastes such as sugarcane molasses as a low-cost sole carbon source.
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