Abstract
AbstractThis study involves the investigation of the complexation ability of poly(2‐[dimethylamino]ethyl methacrylate)‐b‐poly(hydroxypropyl methacrylate) (PDMAEMA‐b‐PHPMA) amphiphilic pH and thermoresponsive block copolymers, and their quaternized counterparts QPDMAEMA‐b‐PHPMA, toward short DNA in aqueous solutions. The PDMAEMA‐b‐PHPMA amphiphilic block copolymers present various self‐assembly characteristics when inserted into aqueous media, depending on the composition, the solubilization protocol, the acidity and the temperature of the aqueous media. Copolymer aggregates‐DNA interactions and nanostructure formation after complexation are investigated by dynamic light scattering and intensity measurements in aqueous solutions in a fixed temperature range, utilizing two different solubilization protocols for the copolymers. Ethidium bromide assays by fluorescence spectroscopy and ζ‐potential measurements were also utilized to investigate the structure and properties of the DNA/copolymer polyplexes. The interpretation of such physicochemical characterization provides extra comprehension of the novel (Q)PDMAEMA‐b‐PHPMA copolymers self‐assembly characteristics and assesses their ability for DNA complexation, stabilization, and delivery.
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