Poly-γ-glutamic acid bioproduct improves the coastal saline soil mainly by assisting nitrogen conservation during salt-leaching process.

Abstract

Salt-leaching is considered to be a major method for soil desalting in agriculture. Therefore, conservation of soil nutrition is significant to soil fertility and environment protection during the salt-leaching process. The effect of poly-γ-glutamic acid bioproduct (PGAB), which was manufactured by solid-state fermentation with the bacteria producing glutamic acid (GA) and poly-γ-glutamic acid (γ-PGA) and organic waste, on keeping nitrogen (N) during salt-leaching was investigated in this study. The isolated bacteria producing GA and γ-PGA were identified as Brevibacterium flavum and Bacillus amyloliquefaciens, respectively. After the saline soil was leached for 90days, compared to the control, soil salinity (0-30cm) in the PGAB treatment was decreased by 39.9%, while soil total N was significantly (P < 0.05) higher than other treatments. Furthermore, the microbial biomass N (0-30cm) in PGAB treatment was increased by 119.5%; populations of soil total bacteria, fungi, actinomyces, nitrogen-fixing bacteria, ammonifying bacteria, nitrifying bacteria, and denitrifying bacteria and soil algae biomass were also significantly (P < 0.05) increased. In terms of physical properties, the percentage of soil aggregates with diameter > 0.25mm was increased by 293.5%, and the soil erosion-resistance coefficient was increased by 50.0%. In conclusion, the PGAB can effectively conserve soil N during the process of salt-leaching and therefore offer a sustainable way to improve coastal saline soil.