Poly(ε-caprolactone)-based substrates bearing pendant small chemical groups as a platform for systemic investigation of chondrogenesis.

Abstract

Physiochemical properties of biomaterials play critical roles in dictating types of cell behaviour. In this study, a series of poly(ε-caprolactone) (PCL)-derived polymers bearing different small chemical groups was employed as a platform to evaluate chondrogenesis of different cell types. Thin films were prepared by spin-coating PCL derivatives. Rabbit articular chondrocytes (rACs) and rabbit bone marrow-derived mesenchymal stem cells (rMSCs) were seeded on to the films, and cell adhesion, proliferation, extracellular matrix production and gene expression were evaluated. The presence of hydrophilic groups (-NH2 , -COOH, -OH and -C=O) promoted adhesion and proliferation of primary rACs and rMSCs. On these polymeric films, chondrogenesis of primary rACs depended on culture time. For passaged cells, re-differentiation was induced on these films by chondrogenic induction, but less for cells of passage 5 compared to passage 3. While films with hydrophilic groups favoured chondrocytic gene expression of both types of passaged cells, production of glycosaminoglycans (GAG) was similar for those of passage 3 on all films, and PCL-CH3 film better supported GAG production for cells of passage 5. Under chondrogenic conditions, rMSCs were more efficient at GAG production on PCL and PCL-NH2 films. This study demonstrates that different cells displayed distinct responses to substrate surface chemistry, implying that cell-biomaterial interactions can be developmental stage dependent. This provides a novel perspective for developing biomaterials for cartilage regeneration.