Pollination Compatibility of Dendrobium Polinela Lampung, and The Effect of Media Composition with Coconut Water on Seed Germination In Vitro

Abstract

Efforts to increase the quality of Dendrobium orchids can be made by increasing the genetic quality through crosses while increasing the quantity can be done by propagation through in vitro culture. Information about the compatibility of an orchid species is very important to maximize the acquisition of seeds as propagation material. The seeds from the cross were propagated by in vitro culture technique. The purpose of this study was to obtain a compatible crossing method between crossing and selfing D.Polinela Lampung orchids with selected parents and to determine the effect of giving a combination of media with coconut water concentration in inducing germination and growth of orchid seeds. This study consisted of two experiments, namely (1) the compatibility test of D.Polinela Lampung orchids with three types of Indonesian orchids (D.discolour, D.leporinum, D.lasientera), and (2) the effect of basic media (MS, VW, Growmore, Gandasil) and water concentration CW (0, 50, 150, and 250 ml/1) on seedling growth of D.Polinela Lampung x D.lasientera orchids. In conclusion, among the sixteen crosses of Dendrobium orchids, ten compatibility crosses were found (pollination that produces fruit). The addition of 15 and 25% CW in the growmore medium (91.37% and 98.03%), MS (90.97% and 96.00 %), and Gandasil (90.67% and 92.67%) gave the highest percentage results. Seed germination and protocorm formation. The protocorm that forms the first leaves produced on Growmore media added with 50-150 ml/1 coconut water gave the most results compared to other treatments. The results of the research are expected as basic information in orchid propagation, especially the study of compatibility and germination of orchid seeds.