Abstract
To the Editor: —The article by Black and Moore (The Journal, January 30) contains statements that are distinctly inaccurate and misleading. F. W. Heyl ( J. Am. Chem. Soc. 39: 1470, 1917) found that ragweed pollen used in his work contained 4.99 per cent of total nitrogen, of which 2.6 per cent was soluble in aqueous saline solution. Of this 2.6 per cent, from 0.6 to 0.8 per cent was protein in nature, part being albumin and proteose. H. L. Huber and K. K. Koessler ( Arch. Int. Med. 36: 751 [Dec.] 1925) obtained solutions of ragweed pollen (1: 10, or 10 per cent) which contained 1.54 and 1.61 mg. of nitrogen per cubic centimeter. According to Heyl's figures, these 10 per cent solutions would contain about 0.5 mg. of protein nitrogen per cubic centimeter, or about 3 mg. of pollen protein per cubic centimeter. Glycerol and water have similar solvent properties.
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