Abstract
This study reports the development of a programmed pollen self-elimination CRISPR-Cas (PSEC) system in which the pollen is infertile whenPSEC is present in haploid pollen. PSEC can be inherited through the female gametophyte and retains genome editing activity invivo across generations. This system could greatly alleviate serious concerns about the widespread diffusion of genetically modified (GM) elements into natural and agricultural environments via outcrossing.
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