Pollen mediated gene flow in a small experimental population of Moringa oleifera Lam. (Moringaceae)

Abstract

Large-scale plantations of Moringa oleifera Lam. are carrying out in the dry-hot valley of Red River in Yunnan Province to make it the core cultivation area in China. Nevertheless, there is a deficient understanding of detailed pollen flow patterns, which leads to uncertainty in designing and managing seed orchards of M. oleifera to fit the breeding demand. In order to facilitate the design of breeding strategies for inbreeding lines, paternity analysis by SSR (Simple Sequence Repeat) was used to estimate the outcrossing rate and pollen mediated gene flow of M. oleifera. The analysis was carried out in an experimental population domesticated in the dry-hot valley area of Red River with two germplasms (India and Myanmar) of 60 trees, including 12 maternal trees and 60 pollen donors (bisexual flowers, maternal trees were also possible paternal trees in case of selfing). One hundred fifteen seeds out of 288 were confirmed pollen donors by 8 microsatellite loci at 95% strict confidence level. The multilocus outcrossing rate is tm = 0.797 ± 0.096, and single-locus outcrossing rate is ts = 0.742 ± 0.058. Pollen dispersal distance varied from 0 m (self-pollination) to 24.7 m, with an average of 11.9 m, and 95% of effective pollen was dispersed within 20 m, indicating that the effective pollen mediated gene flow is restricted to a limited distance. 50.3% of effective pollen was donated by 9 trees. Moreover, there was no apparent correlation between pollen dispersal distance and pollen contribution rate, as well as between germplasm and reproducibility. The maximum pollen dispersal distance between two trees estimated in this experimental stand is highly localized at current 3 × 3 m planting density, indicating that M. oleifera seed orchards and clones could be planted nearer to each other than previously thought (as far as kilometers).