Pollen grain number and viability contribute to variation in effective ovule number among oilseed rape genotypes

Xiao Guo,Bao-Luo Ma,Xiaoming Wu,Yajun Gao,Biyun Chen,Neil B Mclaughlin

doi:10.1007/s10681-021-02954-1

Abstract

While no significant differences in initial ovule number were found among oilseed rape Brassica napus genotypes, there was a large variation in effective ovule number (EON), which determines the final seeds per silique (SPS), a critical component of yield. In this study, we selected 18 oilseed rape Brassica napus genotypes with contrasting nitrogen utilization efficiency (NUtE) to unravel the main factors responsible for different EON and determine the critical period of EON formation under both a field and a pot experiments from 2016–2018. The high NUtE genotypes displayed significantly higher NUtE by 14.3%, along with greater yield per plant (29.4%) and SPS (21.1%) than the low NUtE genotypes. The greater productivity of the high NUtE genotypes was associated with 44.1% higher pollen grain number, 23.5% greater pollen viability, and 39.3% lower ovule abortion rate, compared to the low NUtE genotypes. In addition, the heart stage was the critical ovule development period for delineating the variability of EON among contrasting NUtE oilseed rape Brassica napus genotypes, when the high NUtE genotypes displayed higher silique net photosynthetic rate, surface area, biomass, and RNA expression levels. Taken together, this study indicated the pollen grain number, pollen viability and ovule abortion rate contributed to the final variation in EON and the heart stage was the critical period of determining the EON differences among contrasting NUtE genotypes. Increasing pollen grain number and pollen viability, and decreasing ovule abortion rate before heart stage should be the prerequisite for breeders to improve yield and NUtE of oilseed rape Brassica napus genotypes.

Highlights

Oilseed rape (Brassica napus L.) is the world’s second largest crop source of vegetable oil following soybean, with increasing significance in the international market
This study indicated the pollen grain number, pollen viability and ovule abortion rate contributed to the final variation in effective ovule number (EON) and the heart stage was the critical period of determining the EON differences among contrasting nitrogen utilization efficiency (NUtE) genotypes
Our results showed that the stamens of the contrasting NUtE genotypes were identical and normal in the field and pot experiments (Fig. 1A-D), and no significant differences in anther and filament number were found (Fig. 1E-H, Supplementary Fig. 1)

Summary

Introduction

Oilseed rape (Brassica napus L.) is the world’s second largest crop source of vegetable oil following soybean, with increasing significance in the international market. One of the most important traits in oilseed rape breeding, is the product of seeds per silique (SPS), siliques per plant (SPP) and thousand seed weight (TSW)(Ma et al 2015). The three yield components (SPS, SPP and TSW) of oilseed rape display different degree of negative correlation, which indicates that the final seed yield can be improved by increasing the individual yield components (such as SPS) (Yang et al 2017b). In the study conducted by He et al (2017), the SPS was positively correlated with nitrogen utilization efficiency (NUtE; seed dry weight / shoot N accumulation), which indicated that SPS can be used as a simple and applicable indicator for the evaluation of NUtE in oilseed rape

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Results

Discussion

Conclusion