Abstract
Polio or poliomyelitis is a disabling and life-threatening disease caused by poliovirus (PV). As a consequence of global polio vaccination efforts, wild PV serotypes 2 and 3 have been eradicated around the world, and wild PV serotype 1-transmitted cases have been largely eliminated except for limited regions. However, vaccine-derived PV, pathogenically reverted live PV vaccine strains, has become a serious issue. For the global eradication of polio, the World Health Organization is conducting the third edition of the Global Action Plan, which is requesting stringent control of potentially PV-infected materials. To facilitate the mission, we generated a PV-nonsusceptible Vero cell subline, which may serve as an ideal replacement of standard Vero cells to isolate emerging/re-emerging viruses without the risk of generating PV-infected materials.
Highlights
Polio or poliomyelitis is a disabling and life-threatening disease caused by poliovirus (PV), a nonenveloped, positive-sense single-stranded RNA virus classified in the genus Enterovirus in the family Picornaviridae[1], of which there are three wild serotypes: PV 1–3
The World Health Organization (WHO) GAPIII rationalizes that with the use of inactivated PV vaccines, most countries will have no need to retain live PV in the post-eradication and post-oral polio vaccine (OPV) era, and that facilityassociated risks can be eliminated by destruction of all wild PV and OPV infectious and potentially infectious materials
The Vero cell line is a continuous cell line established from the kidney of the Chlorocebus sabaeus species of African green monkeys (AGMs)[6,7], the whole genome sequences of which have been determined in several sublines[7,8]
Summary
Polio or poliomyelitis is a disabling and life-threatening disease caused by poliovirus (PV), a nonenveloped, positive-sense single-stranded RNA virus classified in the genus Enterovirus in the family Picornaviridae[1], of which there are three wild serotypes: PV 1–3. To provide an ideal replacement of standard Vero cells to isolate emerging/re-emerging viruses without the risk of generating potentially PV-infected materials, we here generated a PV-nonsusceptible Vero cell subline by disrupting genomic genes encoding AGM paralogs of the human PV receptors. The amplified region of the PVR2 gene of Vero cells was found to contain synonymous single nucleotide variations (SNVs) when compared with counterparts in AGM reference sequences (Fig. 1b,c).
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