Abstract
This work aimed to prepare the formulation containing free RuBPY and loaded in PNP and to verify if it induces relaxation in isolated rat aorta. We have analyzed the maximum effect (ME) and potency (pD2). P values 蠄0.05 were considered significant. Vascular reactivity experiments were performed on denuded phenylephrine‐contracted aorta and simultaneous amperometric measurements of NO were performed in time course experiments. It was evaluated the effect of the NO scavengers oxyhemoglobin and hydroxocobalamin on the relaxation induced by the formulation. Formulation containing RuBPY induced relaxation with similar ME (103.5±3.3%) as free RuBPY (100.2±0.7%) and similar pD2 values for formulation (7.15+0.33, n=5) and free RuBPY (7.76+0.20, n=5). Time‐course for free and in formulation RuBPY induced relaxation, and NO released was detected in the solution only by free RuBPY. The NO scavengers reduced the relaxing effect for both with greater inhibitory effect for the formulation. In conclusion, RuBPY formulation is a potential vasodilator and the formulation protects the complex RuBPY avoiding the NO leaking to the solution. Supported by FAPESP and CNPq.
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