Abstract
The half-wave potentials of all-trans beta-carotene, all-trans retinol, 13-cis retinol, all-trans retinyl acetate, all-trans retinal, and vitamins D(2) and D(3) were related to the number of double bonds in conjugation. A minimum of three double bonds in conjugation was required before reduction took place at the dropping-mercury electrode. As the number of conjugated bonds increased in the fat-soluble vitamins, the initial reduction took place at a lower half-wave potential. All of the waves were linearly proportional to the concentration of the vitamins in the concentration range studied.
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