Abstract

Poly(styrene) is a highly viscous, and cross-linked polymer at room temperature. This makes it ideal to use as a molecular fixer. The polarized fluorescence of a diphenylhexatriene (DPH)-incorporated poly(styrene) film has been studied. The excitation and emission wavelength dependence of the anisotropy of fluorescence of a luminophore-incorporated poly(styrene) film reveals that with decreasing excitation wavelength the anisotropy changes remarkably but is independent of emission wavelength. The investigation estimates an angle of no more than 7.4° between the absorption and the emission transition dipole moment for DPH, suggesting poly(styrene) as a suitable medium to evaluate the mutual orientation of absorption and the emission transition dipole moments at room temperature.

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