Polarized and depolarized dynamic light scattering study on F-actin in solution: comparison with model calculations

Abstract

The dynamics of F-actin of different average filament lengths are sudied by polarized and depolarized dynamic light scattering. The experimental data have been compared with model calculations for rigid and semiflexible macromolecules. The polarized DLS spectra follow the predictions for semiflexible rods with a persistence length of (7.5±2.5) μm. Became there is no theory available that includes flexibility in the calculation of the depolarized dynamic structure factor, to a first approximation we used the diffusion coefficients for semiflexible molecules in our rigid-rod model calculations