Polarization-Singular Approach to Imaging Mueller-Matrix Polarimetry in the Differential Diagnosis of Histological Sections of Biopsy of Tumors of the Uterus and Prostate

A Dubolazov,Y Tomka,Y Ushenko,A Ushenko,I Soltys,V Ushenko,V Gantyuk,P Gorodensky,A Stashkevich,L Trifonyuk

doi:10.3389/fphy.2021.711212

Abstract

The possibilities of the diagnostic use of the singular approach of the distributions of the number of characteristic values of the MMI is effective for differentiating the polarization properties of histological biopsy sections of benign and malignant tumours of the uterus and prostate. Within the framework of evidence-based medicine, the sensitivity, specificity and accuracy of the azimuthal-invariant express (∼15 min) method of Mueller-matrix mapping of polarization-singular states in the differential diagnosis of uterine myoma and adenocarcinoma, as well as adenocarcinoma of the prostate with varying degrees of differentiation have been determined.

Highlights

The indicated azimuthal irreproducibility of polarization-singular data is multiplied within the limits of representative samplings of biological samples. To overcome this problem of Stokes polarimetry, we considered the diagnostic capabilities of the polarizationsingular approach using the Mueller-matrix formalism
The indicated oncological destruction of the optical anisotropy of the uterine tissue is accompanied by an increase in the probability of the formation of characteristic values (f 44 ±1) (relations (5), Table 1), which characterize the mechanisms of formation of L—states
3) It was found that the statistical analysis of the distributions of the number of characteristic values of the Mueller-matrix invariant, which characterizes the mechanisms of formation L− and C− states of microscopic images of biological preparations is effective for differentiating the polarization properties of such objects associated with pathological changes in the birefringent fibrillar networks of tumours of the uterus and prostate

Summary

INTRODUCTION

In biomedical optics, polarized light plays a decisive role in understanding and detecting the processes of conversion of electromagnetic waves by optically anisotropic structures of human biological tissues [1,2,3,4,5,6,7,8,9,10,11,12,13,14,15,16,17,18,19,20]. Overcoming this problem can be the use of the Mueller-matrix formalism [10] in the description of the processes of the formation of the polarization-singular structure of the object field of an optically anisotropic biological layer This approach provides unambiguous information on the relationships between the maps of polarization singularities and the parameters of the polycrystalline birefringent structure of fibrillar networks. Μm Attenuation (extinction) coefficient τ, cm−1 Depolarization degree Λ, %

RESULTS

CONCLUSIONS

ETHICS STATEMENT