Polarization Microscopy Imaging of G Proteins at Endogenous Expression Levels

Abstract

G proteins are crucial molecules of many cell signalling processes. Their function has to date been investigated using a wide variety of imaging techniques, including single- and two-photon polarization microscopy (1PPM and 2PPM). Polarization microscopy has allowed examination of the dynamic interactions between GPCRs and G proteins, direct observations of G protein activation, as well as monitoring of downstream signalling events. However, most investigations of G protein signalling by imaging techniques have been carried out in systems overexpressing the protein of interest.