Abstract
AbstractThe polarization assay was used to assess the effects of chemoattractants on neutrophil motility by means of cellular configuration. The addition of N‐formyl‐L‐methionyl‐phenylalanine (f‐Met‐Phe) to a neutrophil suspension from human peripheral blood induced a polarized change in cellular configuration, and the ratio of such transfiguration reached a maximum at 10–8 to 10–6 M of f‐Met‐Phe. The polarized configuration appeared 1 min after addition of f‐Met‐Phe and showed its maximum response at 10 min. The number of polarized cells were decreased after exposure to cytochalasin B or incubating with corticosteroid. The increased response of random polarization was induced in a dose‐dependent fashion by ascorbic acid. Tetracycline HCl did not cause any changes in cell shape at any concentrations examined. This assay can be used to examine patients with depressed neutrophil motility, such as those with systemic lupus erythematosus in the active phase and a severe case of atopic dermatitis.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
