Abstract
Recent experiments revealing nanoscale electrostatic force generation at kinetochores for chromosome motions have prompted models for interactions between positively charged molecules in kinetochores and negative charge at and near the plus ends of microtubules. A clear picture of how kinetochores and centrosomes establish and maintain a dynamic coupling to microtubules for force generation during the complex motions of mitosis remains elusive. The molecular cell biology paradigm requires that specific molecules, or molecular geometries, for polar force generation be identified. While progress has been made regarding explanations of kinetochore-based chromosome motility, molecular machinery for chromosome poleward movements at centrosomes has yet to be identified. The present work concerns polar generation of poleward force in terms of experimentally known electric charge distributions at microtubule minus ends and centrosomes interacting over nanometer distances.
Highlights
Current thought on mitotic motions is being considered in a more electrostatics-based framework [1], corroborating theoretical predictions made a decade ago [2,3]
We propose that nanoscale electrostatic interactions between microtubule minus ends and charge distributions at centrosomes are responsible for polar generation of force for poleward chromosome motility during mitosis
To minus-ends of microtubules in any way – for poleward force production at centrosomes.The model proposed here is restricted to polar generation of poleward force for chromosome motility, an aspect of mitosis for which there is no established molecular biology paradigm
Summary
Current thought on mitotic motions is being considered in a more electrostatics-based framework [1], corroborating theoretical predictions made a decade ago [2,3]. Microtubules continually assemble and disassemble, so the turnover of tubulin is ongoing. The rate at which microtubules undergo net assembly – or disassembly – varies with mitotic stage [5]. We propose that nanoscale electrostatic interactions between microtubule minus ends and charge distributions at centrosomes are responsible for polar generation of force for poleward chromosome motility during mitosis. Kinetochore minus-end disassembly at poles associated with poleward microtubule flux is known to produce a force that can do. To minus-ends of microtubules in any way – for poleward force production at centrosomes (poles).The model proposed here is restricted to polar generation of poleward force for chromosome motility, an aspect of mitosis for which there is no established molecular biology paradigm
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
