Point Spread Function of Objective-Based Surface Plasmon Resonance Microscopy.

Abstract

Objective-based surface plasmon resonance microscopy (SPRM) is a novel optical imaging technique that can map the spatial distribution of a local refractive index based on propagating surface plasmon polaritons (SPPs). Different from some other optical microscopy that shows a dot-like point spread function (PSF), a nanosized object appears as a wave-like pattern containing parabolic tails in SPRM. The geometrical complexity of the wave-like pattern hampered the quantitative interpretation of the PSF of SPRM. Previous studies have shown that two adjacent rings were obtained in the frequency domain by applying a two-dimensional Fourier transform to such patterns. In the present work, a ring-fitting method was developed to extract geometrical features out of the dual rings and to connect these features with several experimental parameters. It was found that the radius of ring equaled to the wavevector of SPPs. Its orientation revealed the propagation direction of SPPs. The coordinate distance of the center of ring gave the parallel component of the wavevector of the incident light, which was regulated by the incident angle. The ring-broadening factor reflected the propagation length of SPPs in a reciprocal relationship. Systematical and quantitative interpretations in the frequency domain not only advanced the basic understanding on the PSF of SPRM but also opened up the possibility to utilize these frequency-domain features for detection and sensing purposes in future.