Pod Trichome Characterisation Using Foldscope, Morphological Characterization and Genetic Diversity Among Indigenous Collections of Pigeonpea [Cajanus cajan (L.) Millsp.

Abstract

Background: Association of several morphological traits such as trichome length and trichome density on the pod surface have been found to influence host plant resistance to insect pests. Genetic diversity analysis is used to identify the divergent genotypes and to utilize these genotypes to exploit heterosis. Further, morphological characters are stable across environments owing to oligogeneic nature, they serve as morphological markers in breeding which can be used in varietal or genotypic identification, varietal purification and even in seed production. Hence, the current study aimed to investigate on host plant resistance using portable paper microscope ‘foldscope’, genetic diversity and morphological characters.Methods: A total of 154 germplasm lines with three checks were evaluated in Augmented Block Design (ABD) at Zonal Agricultural Research Station (ZARS), Kalaburagi, during kharif, 2018 to study their genetic diversity. Trichome length and trichome density were recorded using ‘foldscope’ 75 randomly selected genotypes, then correlated with shrivelled seed yield per plot due to pod fly incidence. Morphological characterization of 14 qualitative traits were recorded.Result: Grouping of 157 germplasm lines into twelve clusters indicated a wider genetic diversity for the traits studied, of which 7 clusters were solitary with one entry each. The genotypes with more trichome density and length had less damage by the pod fly. Large variations for morphological characters was observed among the genotypes for qualitative traits such as pod colour, stem colour, flower colour, seed morphology and pod trichomes.