Abstract
In mammals, Clusterin (Clu) is a pleiotropic glycoprotein associated with various physiological and pathological functions, and plays significant roles in innate immunity. However, immune function of Clu in teleost is essentially unknown. In this study, we studied the complement activation and antibacterial functions of Japanese flounder (Paralichthys olivaceus) Clu (PoClu). PoClu contains two conserved CLα and CLβ domains, and shares 30.46%–70.28% sequence identities with other Clu. We found that PoClu expression occurred in various tissues and regulated by bacterial stimulation. Recombinant PoClu (rPoClu) bound both gram-positive and -negative bacteria and sheep red blood cell, but failed to affect bacterial growth in vitro. rPoClu could significantly suppress the bactericidal and hemolysis activity of Japanese flounder serum. Consistently, the bactericidal and hemolysis activity of fish serum were enhanced by anti-rPoClu antibody, further confirmed the negative role of PoClu in complement activity. Furthermore, In vivo rPoClu also exhibited the negative role in host against bacterial dissemination. In conclusion, our results provide the first evidence to unveil that teleost Clu could play a negative role in complement activation and host against bacterial infection.
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