PO311 EVALUATION OF PROMISING PLANT EXTRACTS AND ACTIVE CONSTITUENTS FOR ANTI OBESE, ANTI DIABETIC AND HEPATOPROTECTIVE PROPERTIES IN WNIN/GR-OB RATS

Abstract

Background: Recently, skeletal muscle is recognized as an endocrine organ releasing myokines. These myokines have been shown to mediate not only health-promoting effects of physical exercise but also act favorably on insulin resistance, metabolic dysfunction, and inflammation. This study examined various myokines in patients with type 2 diabetes and pre-diabetes and compared these with normal healthy control, and assessed how they are related to various metabolic parameters, body composition, vascular stiffness and atherosclerosis. Method: One hundred forty prediabetes or diabetes patients and normal healthy controls participated in a crosssectional study. Fasting plasma glucose, HbA1c, lipid profile, insulin sensitivity index, electrical bioimpedence, pulse wave velocity, and carotid intima-media thickness (IMT) were measured. Serum irisin, IL-13, IL-6, FGF-21, fractalkine, and follistin-like 1 (FSTL1) were measured using enzyme-linked immunosorbent assay method. Result: There were significant differences in serum irisin, IL-13, IL-6, fractalkine, and FSTL1 level among normal, prediabetes, and diabetes groups. Serum irisin, IL-13, and FSTL1 levels were higher in pre-diabetes group and lower in diabetes group compared to control (P< 0.05). IL-6 and fractalkine increased gradually in the order from normal to pre-diabetes to diabetes group, and it was significantly higher in diabetes group compared to other two groups (P< 0.01). Irisin, IL-13, were in negative correlations with HbA1c, fasting glucose, 1 and 2-hour glucose (all P< 0.01) while IL-6 and fractalkine were in positive correlations (P< 0.01) after adjusting for age and gender. IL-13 showed a significant negative correlation with right mean carotid intima media thickness (IMT) after adjusting for age and gender (P< 0.05). In a multivariable model adjusted for various metabolic parameters, subjects in highest tertile of irisin levels were less likely to have type 2 diabetes compared to those in the lowest tertile (odds ratio (OR) = 0.64, 95% confidence interval (CI) 0.47– 0.88). Furthermore, multiple regression analysis showed that serum irisin level was an independent predictor of 2h plasma glucose and HbA1c level (p= 0.004). Conclusion: Various myokines including irisin, IL-6, IL-13, FSTL1, and fractalkine showed close relationships with blood glucose level in subjects within the spectrum of normal, prediabetes, and diabetes states. Irisin, IL-13, and FSTL1 seem to have a compensatory increase in prediabetes stage followed by a drop in overt diabetes stage, and they were independent predictors of the type 2 diabetes. Further study is warranted to elucidate their roles in glucose metabolism.