PO-160 Effect of exercise intervention on the synaptic transmission efficiency of cortical-striatum in PD mice

Abstract

Objective Parkinson's disease (PD) is a neurodegenerative disease caused by degeneration of dopaminergic neurons in the substantia nigra pars compacta and decreased levels of striatal dopamine. Previous studies have confirmed that striatum dysfunction is an important cause of behavioral dysfunction in PD mice, and changes in cortical-striatum synaptic transmission may play an important role in PD pathology. This experiment will use the patch clamp to explore the effect of exercise intervention on the synaptic transmission efficiency of cortical-striatum in PD mice.
 Methods The experimental animals (C57 mice male, 4 weeks) were randomly divided into normal group(N), PD group(PD)and PD exercise group(PD+EX）.PD mice models were established by injection of 6-OHDA in the striatum (4μg/position, AP + 0.5mm ML - 1.8mm DV - 3.0mm and- 2.0mm). Apomorphine(APO) rotation experiments were performed one week later to identify the model. The formal exercise intervention program was 16m/min, 40min/d, 5d/week,4 weeks（after the adaptive training, total of five weeks of treadmill exercise）. At the end of the intervention, brain slices (350μm) were prepared from each group of mice. The maximum striatum fEPSP (field excitatory postsynaptic potential, fEPSP) and striatum fEPSP under increasing stimulation intensity were recorded.
 Results 1.The results of APO rotation experiment: PD mice modeled 16/21(205.25±36.46 r higher than the standard). 2.N mice the maximum fEPSP amplitude is 1.9199±0.213mv(n=8)；PD: 1.0129±0.140 mv(n=8); PD+EX: 1.3221±.0865mv(n=8).There are significant differences between groups(P＜0.05). 3. There is a significant decrease in the slope of I-O curve in the PD mice compared with the N mice. And compared with the PD mice, the slope of I-O curve in PD+EX mice was increase(P＜0.05).
 Conclusions 1. 6-OHDA induced PD model was successfully established. The striatum function was abnormal in the pathological state of PD, and the exercise intervention enhanced the activity of the striatum. 2. The cortical-striatum synaptic transmission efficiency is reduced in the PD state, and the exercise intervention improved the cortical-striatum synaptic transmission efficiency.