PO-305 An 8-week, low carbohydrate, high fat, ketogenic diet enhanced exercise capacity through improved ketolysis and lipolysis in mice

Sihui Ma,Katsuhiko Suzuki,Qingyi Huang,Chunhong Liu,Takaki Tominaga

doi:10.14428/ebr.v1i5.13383

Abstract

PO-305 An 8-week, low carbohydrate, high fat, ketogenic diet enhanced exercise capacity through improved ketolysis and lipolysis in mice

Highlights

It is reported by Amati and colleagues that well-trained athletes exhibit higher levels of intramuscular triacylglycerol (IMTG) and diacylglycerol (DAG) as well as well-preserved sensitivity to insulin, indicating lipolysis ability may be enhanced during exercise
The best explanation is that the well-adaption of lipid-centered metabolism, including metabolic flexibility and increased IMTG reservoir weakened the need to pull the trigger; this may be the answer why ketogenic diet (KD) mice had lower weight. 3.2 Fatty acid mobilation related RNA alternation after exhaustive exercise under endogenous ketosis in epididymal adipose tissue Adipose triglyceride lipase (ATGL) is known as desnutrin in the first place, is a kind of lipase whose substance is patatin-like phospholipase domain-containing protein
ATGL and Hormone-sensitive lipase (HSL) cooperated to break apart fatty acids from TG, after which IMTG-origin fatty acid will be directly used for beta-oxidation, or lipid drop-origin fatty acids will be transported though lipoprotein shipping in the form of VLDL from adipose tissue into muscle fibers during exercise

Summary

Objective

Carbohydrates, lipids and proteins are utilized both for energy production and structure of body. Protein is the most important component of our body, carbohydrates and lipids are more flexible for energy supply system. Due to carbohydrate pitfall and lipid reserve abundance, coaches and elite athletes aspirate for an effective way to enhance fat utilization. Intramuscular triacylglycerol (IMTG) is a special way for skeletal muscle to store lipids. Intramuscular lipolysis regulation is crucial for energy supply system during exercise. It is reported by Amati and colleagues that well-trained athletes exhibit higher levels of IMTG and diacylglycerol (DAG) as well as well-preserved sensitivity to insulin, indicating lipolysis ability may be enhanced during exercise. We reported that an 8-week, a low carbohydrate, ketogenic diet increased running time till exhausted in male C57BL6/J mice, presuming the mechanism to be enhanced fat utilization. We observed the alternation pattern of messenger RNAs related to lipid mobilization, fatty acid utilization and ketone body oxidation in muscle and adipose tissue immediately after exercise in both Type 1 and Type 2 muscle fibers

Materials and Methods

Findings

Conclusions