PO-179 Breast cancer cells and macrophages in a paracrine-juxtacrine loop

Abstract

IntroductionCancer metastasis is a main cause for mortality. Dysregulation of pericellular proteolysis usually accounts for cancer cell invasion and metastasis. In this study, we are interested in delineating the role of hepatocyte growth factor activator inhibitor-2 (HAI-2) in prostate and lung cancer cell invasion and metastasis.Material and methodsWe used different prostate and lung cancer cells and animal models to examine the role of HAI-2 in prostate and lung cancer cell invasion and metastasis.Results and discussionsIn this study, we identified a membrane-anchored serine protease inhibitor, hepatocyte growth factor activator inhibitor-2 (HAI-2), was down-regulated in human prostate and lung cancer. The decreased levels of HAI-2 were related to the progression of human prostate and lung adenocarcinoma. HAI-2 overexpression can repress both cancer cell migration and invasion. Recombinant HAI-2 proteins can inhibit both cancer cell motility. In addition, HAI-2 overexpression reduced the tumour growth and metastasis of prostate cancer cells, while down-regulation of HAI-2 can increase the metastatic ability of lung adenocarcinoma cells in xenografted animal models.ConclusionHAI-2 functions as a suppressor to inhibit the cell invasion and metastasis of human prostate and lung cancer.