PO-057 Genistein, a major isoflavone component, suppresses Src-induced proliferative activity by arresting cell cycle at G2/M through increasing the P53 and P21 levels

Abstract

IntroductionZe-Qi-Tang (ZQT) is a classic Chinese herbal formula, consisting of nine different herbs, which has been used to effectively cure respiratory tract diseases for thousands of years.In the present study, we aimed to elucidate the anti lung cancer effect of ZQT in tumor-bearing mouse and the underlying antineoplastic mechanism of action.Material and methodsCCK-8 and colony formation assay were used to investigate the cell growth. Flow cytometry analysis was used to evaluate the cell cycle and cell apoptosis. The peroxide-sensitive fluorescent probe DCFH-DA was used to measure the intracellular ROS levels. Western blot assay was used to detect the levels of cell cycle and apoptosis related proteins.Xenografts in nude mice were used to evaluate the effect of ZQT on lung cancer cell in vivo.ELISA assay was test the liver and kidney function post ZQT treatment. Results and discussionsLung cancer cells were significantly killed by ZQT,and it inhibited the proliferation of lung cancer cells and induced cell cycle arrest at S phase and mitochondrial-related apoptosis. Moreover, ZQT induced a sustained activation of the phosphorylation of ERK and JNK. Moreover, ZQT provoked the generation of reactive oxygen species (ROS) in lung cancer cells.In vivo, ZQT suppressed tumour growth in mouse xenograft models.Besides, ZQT was safe, showing minimum toxicity in liver and kidney.ConclusionThese findings suggest Traditional Chinese medicine Ze-Qi-Tang formula is promising to be a novel, potent and safe anti tumour drug candidate for lung cancer.