PO-036 Traditional chinese medicine Ze-Qi-TANG formula induces apoptosis and S phase arrest via ROS-dependent JNK and ERK activation in lung cancer

Abstract

IntroductionDespite being significant advances in colon cancer treatments, recurrence and chemoresistance remain an important challenge in the treatment of patients. During the process of autophagy, cancer cells acquire anoikis resistance and escape chemotherapy. The High Mobility Group Box 1 (HMGB1) molecule is a key mediator of autophagy and can be exploited to develop effective targeted anticancer therapies. Glycyrrhizin and quercetin are natural inhibitor of high mobility box 1 protein (HMGB1).We studied the anticancer activity of glycyrrhizic acid and quercetin in SW480, HT29 and DLD1 colorectal adenocarcinoma cells using alamar blue and trypan blue exclusion assay and determined their IC50. We also proposed to illustrate the anticancer mechanism of these natural compounds by studying cell cycle response, proteome profile and response of cell cycle specific genes in HT29 and DLD1 colorectal cancer cells. natural inhibitor of high mobility box 1 protein (HMGB1).We studied the anticancer activity of glycyrrhizic acid and quercetin in SW480, HT29 and DLD1 colorectal adenocarcinoma cells using alamar blue and trypan blue exclusion assay and determined their IC50. We also proposed to illustrate the anticancer mechanism of these natural compounds by studying cell cycle response, proteome profile and response of cell cycle specific genes in HT29 and DLD1 colorectal cancer cells.Material and methodsCell cycle analysis was performed using a propidium iodide based staining assay in a Muse flow cell analyzer. For proteomics response, R and D proteome-profiler-antibody-arrays for oncology panel for 84 oncoproteins were assayed. A SYBR green based approach for the quantification of selected genes was considered in qPCR analysis.Results and discussionsBoth glycyrrhizic acid and quercetin showed altered protein and gene expression in HT29 and DLD colorectal adenocarcinoma cells.ConclusionAs evident from their low IC50s (80 and 160mcg/ml) against the colorectal cells, both quercetin and glycyrrhizic acid showed a promising scaffold for medicinal chemistry to advance anticancer drug discovery.