PO-023 The Effects of Aerobic Exercise on Alternative Splicing of PKC δI pre-mRNA

Abstract

Objective Alternative splicing of genes is the main way to produce large numbers of proteins, but the mechanism is unclear. The aim of this study was to evaluated the effect of aerobic exercise on PKC δI pre-mRNA alternative splicing. Further, to explore the effect of aerobic exercise on SFRS10 concentration. Because the PKCδ1 is involved in the regulation of adipocyte differentiation and splice factor SFRS10 regulates alternative of PKCδ1, explore the mechanism of PKCδ1 alternative splicing, understand the role of the alternative splicing variants, to provide the theory basis for the mechanism of aerobic exercise reduce the incidence of obesity.
 Methods C57BL/6 male mice were randomly divided into normal quiet group, normal exercise group, obese and quiet group, and obese exercise group. The exercise group performed aerobic exercise for 8 weeks. The intensity of aerobic exercise was: running platform slope is 0, speed 10 m/min, 1 h/time, 1 time/day, 6 times/week for a total of 8 weeks. Immediately after exercise, the cDNA was extracted from liver and adipose tissue. The contents of PKCδ1 and SFRS10 in liver and adipose tissue were determined by PCR and RT-PCR. Liver and fat were stained by oil red O staining to observe lipid droplet changes. And the mouse's Lee's index and blood lipids were determined.
 Results Lee's index = 3√ (body weight * 1000) / body length, Lee's index of obese mice decreased significantly after aerobic exercise, in addition, after aerobic exercise, total cholesterol (TC), triglyceride (TG) and low density Lipoprotein cholesterol (LDL-C) also showed a downward trend (P < 0.05), while high-density lipoprotein cholesterol (HDL-C) increased (P < 0.05); oil red O staining results showed lipid droplets become smaller after aerobic exercise. The results of PCR and RT-PCR showed in the obese and quiet group than in the normal quiet group, the content of PKCδ1-FL decreased, the content of PKCδ1-△Exon9 increased, and the content of SFRS10 decreased. In the normal exercise group than in the normal quiet group and in the obese exercise group than in the obese and quiet group, the PKCδ1-FL content increased, the PKCδ1-△Exon9 content decreased, and the SFRS10 content increased.
 Conclusions Aerobic exercise can significantly increase the content of PKCδ1-FL and SFRS10. PKCδ1-FL inhibits the formation of adipocytes, SFRS10 promotes the inclusion of PKCδ1 exon 9, and there is a molecular mechanism of alternative splicing between PKCδ1 and SFRS10.