Abstract
Leucine-rich repeats receptor-like kinases (LRR-RLKs) play important roles in plant growth and development as well as stress responses. Here, 56 LRR-RLK genes were identified in the Antarctic moss Pohlia nutans transcriptome, which were further classified into 11 subgroups based on their extracellular domain. Of them, PnLRR-RLK27 belongs to the LRR II subgroup and its expression was significantly induced by abiotic stresses. Subcellular localization analysis showed that PnLRR-RLK27 was a plasma membrane protein. The overexpression of PnLRR-RLK27 in Physcomitrella significantly enhanced the salinity and ABA tolerance in their gametophyte growth. Similarly, PnLRR-RLK27 heterologous expression in Arabidopsis increased the salinity and ABA tolerance in their seed germination and early root growth as well as the tolerance to oxidative stress. PnLRR-RLK27 overproduction in these transgenic plants increased the expression of salt stress/ABA-related genes. Furthermore, PnLRR-RLK27 increased the activities of reactive oxygen species (ROS) scavengers and reduced the levels of malondialdehyde (MDA) and ROS. Taken together, these results suggested that PnLRR-RLK27 as a signaling regulator confer abiotic stress response associated with the regulation of the stress- and ABA-mediated signaling network.
Highlights
Land plants are constantly challenged by environmental stresses such as drought, salinity and extreme temperature, which can cause irreversible damage to plants intracellular structures by severe dehydration [1]
These LRR-receptor-like kinases (RLKs) genes were sequential named from PnLRR-RLK1 to PnLRR-RLK56 by their E-value of the Protein kinase domain (PF00069) which generated by Hmmsearch program (Fig 1)
The PnLRR-RLK27 was relatively close to Arabidopsis and Oryza sativa LRR II family proteins which were reported to be induced by various stresses (Fig 1) [46]
Summary
Land plants are constantly challenged by environmental stresses such as drought, salinity and extreme temperature, which can cause irreversible damage to plants intracellular structures by severe dehydration [1]. Plant internal alterations in response to environmental signals mostly depend on a sophisticated signaling network. Membrane anchored receptor-like kinases (RLKs) are the key regulators to active such signaling pathways by perceiving and processing external stimuli to cellular signaling molecules [2]. Pohlia nutans PnLRR-RLK27 positively regulates salinity and oxidation-stress tolerance design, data collection and analysis, decision to publish, or preparation of the manuscript
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