PNIPAAm-based biohybrid injectable hydrogel for cardiac tissue engineering

Abstract

Event Abstract Back to Event PNIPAAm-based biohybrid injectable hydrogel for cardiac tissue engineering Ali Navaei1, Danh Truong1, John Heffernan1, Josh Cutts1, David Brafman1, Rachael Sirianni1, 2, Brent Vernon1 and Mehdi Nikkhah1 1 Arizona State University, School of Biological and Health Systems Engineering, United States 2 Barrow Neurological Institute, Barrow Brain Tumor Research Center, United States Injectable biomaterials offer a non-invasive method to deliver cells into the myocardial infarct region to maintain an adequate level of cellular retention and viability and promote the regeneration process. However, conventional injectable matrices suffer from either low bioactivity or poor mechanical properties. To that extent, in this work we introduced a biohybrid temperature-responsive PNIPAAm-Gelatin-based injectable hydrogel, with excellent bioactivity and mechanical robustness for cardiac tissue engineering. A unique feature of our work was performing extensive in vitro biological analyses to assess the functionalities of cardiomyocytes (CMs) alone and in co-culture with cardiac fibroblasts (CFs) within the hydrogel matrix. The synthesized injectable hydrogel exhibited suitable viscoelastic behavior and adequate water content to properly accommodate the cardiac cells. The encapsulated cells demonstrated a high level of survival and spreading throughout the matrix in both culture groups. A dense network of F-actin fibers illustrated the formation of an intact and three dimensional (3D) cardiac tissue construct within the hydrogel matrix. Furthermore, immunostaining images revealed typical phenotypic characteristics in cardiac cells. Interestingly, the co-culture group exhibited significant improvements in structural organization, cell-cell coupling, genes expression, as well as beating behavior. This study opens a new avenue for extensive in vitro characterizations of injectable matrices, using mono- and co-culture of cardiac cells, prior to in vivo experiments. Keywords: Hydrogel, Regenerative Medicine, MYOCARDIAL TISSUE, Cell functionality Conference: 10th World Biomaterials Congress, Montréal, Canada, 17 May - 22 May, 2016. Presentation Type: New Frontier Oral Topic: Biomaterials in constructing tissue substitutes Citation: Navaei A, Truong D, Heffernan J, Cutts J, Brafman D, Sirianni R, Vernon B and Nikkhah M (2016). PNIPAAm-based biohybrid injectable hydrogel for cardiac tissue engineering. Front. Bioeng. Biotechnol. Conference Abstract: 10th World Biomaterials Congress. doi: 10.3389/conf.FBIOE.2016.01.02417 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 27 Mar 2016; Published Online: 30 Mar 2016. Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Ali Navaei Danh Truong John Heffernan Josh Cutts David Brafman Rachael Sirianni Brent Vernon Mehdi Nikkhah Google Ali Navaei Danh Truong John Heffernan Josh Cutts David Brafman Rachael Sirianni Brent Vernon Mehdi Nikkhah Google Scholar Ali Navaei Danh Truong John Heffernan Josh Cutts David Brafman Rachael Sirianni Brent Vernon Mehdi Nikkhah PubMed Ali Navaei Danh Truong John Heffernan Josh Cutts David Brafman Rachael Sirianni Brent Vernon Mehdi Nikkhah Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.