PMMA/paper hybrid microfluidic chip for simultaneous determination of arginine and valine in human plasma.

Abstract

Thesimultaneous determination is reported of arginine (Arg) and valine (Val) amino acids in plasma using flower-shaped μPADs and PMMA/paper hybrid microfluidic chip based on AuNPs capped with R-thiazolidine-4-carboxylic acid (THP). In this article, the evaluation procedure is based on the smartphone colorimetric detection mechanism that results from the aggregation of the THP-AuNPs with the addition of amino acids and visual color change from red to blue. Arg and Val were selectively determined with good reproducibility and an acceptable linearity range. The flower-shaped (μPADs) provides many advantages, including low cost, reasonable sensitivity, simple and fast performance, simultaneous detection, disposable use, and high sample throughput compared with conventional colorimetric method using cuvette cells. The ratios between the absorbance wavelength at (A650/A525) and (A685/A525) are linearly proportional to the concentration of Arg and Val. Under the optimum conditions, the calibration range in aqueous solutions is 0.0068-100.0 and 0.0056-75.0µM with a limit of detection of 2.25 and 1.86nM for Arg and Val at pH 7.0, respectively. In the case of μPADs, the calibration curves for Arg and Val showed good linearity in the concentration range 0.01-75.0µM. The detection limits for the analytes were 3.51nM and 3.44nM for Arg and Val, respectively. In addition, a PMMA/paper hybrid microfluidic chip was successfully employed to determine Arg and Val in plasma samples with a relative error below 5%.