PM 7/124 (1) Spodoptera littoralis, Spodoptera litura, Spodoptera frugiperda, Spodoptera eridania

Abstract

Contents Specific scope 410 1. Introduction 411 2. Identity 411 3. Detection 411 4. Identification 412 4.1. Morphological identification 412 4.1.1. Eggs 412 4.1.2. Larvae 412 4.1.2.1. Introduction 412 4.1.2.2. General description Spodoptera larvae 413 4.1.2.3. Spodoptera littoralis and S. litura 414 4.1.2.4. Spodoptera eridania 417 4.1.2.5. Spodoptera frugiperda 419 4.1.2.6. Possible confusion with Spodoptera exigua 421 4.1.3. Pupae 422 4.1.3.1. General description 422 4.1.3.2. Possible confusion with Spodoptera exigua 422 4.1.4. Adults 422 4.1.4.1. Introduction 422 4.1.4.2. Spodoptera littoralis 424 4.1.4.3. Spodoptera litura 425 4.1.4.4. Spodoptera eridania 427 4.1.4.5. Spodoptera frugiperda 429 4.2. Molecular methods 430 5. Reference material 430 6. Reporting and documentation 430 7. Performance criteria 430 8. Further information 431 9. Feedback on this diagnostic protocol 431 10. Protocol revision 431 11. Acknowledgements 431 12. References 431 Appendix 1: Features of Lepidoptera larvae 433 Appendix 2: Keys to adults based on external morphology 434 Appendix 3: Information on genitalia 435 Appendix 4: Identification of Spodoptera eridania and S. frugiperda using real‐time PCR 441 Appendix 5: Identification of Spodoptera littoralis and S. litura using real‐time PCR 442 Specific scopeThis Standard provides guidance for the identification of S. littoralis, S. litura, S. frugiperda and S. eridania.Specific approval and amendmentThis Standard was approved in 2015‐09.