PlWRKY13: A Transcription Factor Involved in Abiotic and Biotic Stress Responses in Paeonia lactiflora.

Xue Wang,Junjie Li,Yan Ma,Jing Guo,Qian Qiao,Xianfeng Guo

doi:10.3390/ijms20235953

Xue Wang, Junjie Li + Show 4 more

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https://doi.org/10.3390/ijms20235953

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Abstract

Many members of the WRKY family regulate plant growth and development. Recent studies have shown that members of the WRKY family, specifically WRKY13, play various roles in the regulation of plant stress resistance. To study the function of WRKY family members in peony, the PlWRKY13 gene (KY271095) was cloned from peony leaves. Sequence analysis and subcellular localization results revealed that PlWRKY13 has no introns, belongs to the type IIc subgroup of the WRKY family, and functions in the nucleus. The expression pattern of PlWRKY13 was analysed via real-time quantitative RT-PCR (qRT-PCR), which showed that the expression of PlWRKY13 was induced by four types of abiotic stress, low-temperature, high-temperature, waterlogging and salt stress, and was positively upregulated in response to these stresses. In addition, the expression of PlWRKY13 tended to first decrease and then increase after infection with Alternaria tenuissima. Virus-induced gene silencing (VIGS) technology was used to explore the function of PlWRKY13 in the resistance of Paeonia lactiflora to fungal infection further, and the results showed that PlWRKY13-silenced plants displayed increased sensitivity to A. tenuissima. The infection was more severe and the disease index (DI) significantly greater in the PlWRKY13-silenced plants than in the control plants, and the expression of pathogenesis-related (PR) genes was also significantly altered in the PlWRKY13-silenced plants compared with the control plants. The contents of the endogenous hormones jasmonic acid (JA) and salicylic acid (SA) were measured, and the results showed that the JA content increased gradually after infection with A. tenuissima and that JA may play an active role in the resistance of P. lactiflora to pathogen infection, while the SA content decreased after PlWRKY13 silencing. The contents of the two hormones decreased overall, suggesting that they are related to the transcription of PlWRKY13 and that PlWRKY13 may be involved in the disease-resistance pathway mediated by JA and SA. In summary, the results of our study showed that PlWRKY13 expression was induced by stress and had a positive effect on the resistance of P. lactiflora to fungal infection.

Highlights

Organisms evolve to adapt to their environment, which is the case for plants despite being sessile
The results showed that the PlWRKY13 open reading frame (ORF) was 693 bp long and encoded 230 amino acids
The results showed that PlWRKY13 and AtWRKY13 were a branch of the type IIc subgroup of the WRKY family

Summary

Introduction

Organisms evolve to adapt to their environment, which is the case for plants despite being sessile. Plants have evolved defence mechanisms that respond to environmental stress. These mechanisms strongly depend on the correct perception and transduction of signals through signalling cascades; plant defence responses are regulated by signalling networks [1]. The transcriptional regulation of defence-related genes that respond to stress plays an important role in the development of plant stress tolerance. Several specific DNA-binding TF families that regulate the expression of plant defence-related genes have been identified, including ERF, NAC, MYB, bZIP and WRKY proteins that bind to W-box elements [3]. WRKYs are of particular interest because they are involved in a variety of biotic and abiotic stress responses in plants and in a variety of growth and development processes. WRKYs are highly specific and exert their regulatory activity strictly by binding to downstream promoter elements; these TFs are expected to be candidate genes for crop improvement [4]

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