Abstract
Aim: Abnormal prostate growth is the most prevalent pathological sign in aged human males, reflected by high incidence of Benign Prostatic Hyperplasia (BPH) and Prostate Cancer (PCa). The successful isolation and cultivation of prostate cells, is a prerequisite need for establishing model cell line for understanding the pathogenesis, unique biological properties and also various evidences suggest role of stem cells in pathogenesis of these conditions for therapeutic point of view. Methods and results: Here we isolate a candidate pluripotent stem cell population from BPH patients underwent TURP which include the isolation of an enriched population of prostate stem cells through cell culture techniques and the cultivation of prostate stem cells in vitro and characterization of these cells and their stem potential, including in-vivo teratoma generation. Cytogenetic analysis by G-banding assay demonstrated an aneuploid karyotype with a model chromosome number of 60 and normal Y chromosome. Characterization of isolated cells showed the presence of ONS pluripotency stem cell markers. Beside this these cells were also found positive for stem cell surface markers such as CD49b, CD44, CD117, CD34 and prostatic tissue specific markers like p63 and Androgen Receptor. In-vitro differentiation of the cells demonstrated formation of a tri-germinal layer into ectodermal, endodermal and mesodermal cell lineages with defined medium conditions and In-vivo teratoma formation in excised tumor in Balb/c mouse. Conclusion: we report here isolation, establishment and characterization of human prostate-derived pluripotent stem cell line. The cell line eventually serves as a potential tool for studies in prostate adult stem cell research, understanding etiopathophysiology and the regulation of BPH and PCa.
Highlights
The prostate is a hormonally regulated organ whose growth accelerates at sexual maturity due to androgen actions on both stromal and epithelial cells
Conclusion: we report here isolation, establishment and characterization of human prostate-derived pluripotent stem cell line
benign prostate hyperplasia (BPH) is a slow progressive enlargement of the prostate gland which can lead to lower urinary tract symptoms (LUTS) in elderly men
Summary
The prostate is a hormonally regulated organ whose growth accelerates at sexual maturity due to androgen actions on both stromal and epithelial cells. Unavailability of normal/ benign prostate cell lines and suitable animal model made these attributes difficult to study in vitro and in vivo. Human prostate contains mainly two types of cells, epithelial and stromal cells. The stromal to epithelial ratio in normal prostate of human is 2:1 [3,4]. The basal cells form a layer of flattened to cuboidal shaped cells above the basement membrane and express p63 (a homolog of the tumor suppressor gene p53), Bc1-2 (an antiapoptotic factor), Cluster designation (CD) 44, hepatocyte growth factor (HGF), and the high molecular weight cytokeratins (CK) 5 and 14.The expression of androgen receptor (AR) is low or undetectable in the basal cells, which makes the basal cells independent of androgens for their survival [5,6,7]
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