Plumericin inhibits the viability of cervical cancer cells by induction of apoptosis through degradation of DNA structure

Jing Sun,De-Qing Sun,Rong-Mei Wang,Qi-Yue Wan,Li-Ci Wang,Li Zhang,Yao-Yang Liu,Jing Wu

doi:10.3329/bjp.v11i2.24918

Abstract

<p>The current study demonstrates the effect of plumericin on the viability and induction of apoptosis through degradation of double helical DNA structure in Bu25TK and HeLa cervical carcinoma cell lines. Plumericin treatment exhibited concentration- and time-dependent effect on the viability of Bu25TK and HeLa cells. Incubation of Bu25TK and HeLa cells with 40 µM concen-tration of plumericin decreased cell viability to 24 and 29%, respectively after 48 hours. Plumericin treatment also caused significant increase in the proportion of apoptotic cells in both the cell lines at 40 µM concentration compared to the control cells. Examination of the DNA double helical structure after 48 hours of treatment with plumericin at 40 µM concentration showed DNA degradation and formation of comets. Thus, plumericin exhibits inhibitory effect on the viability of Bu25TK and HeLa cells and induced apoptosis through DNA degradation. Therefore, plumericin is a potent candidate for the treatment of cervical carcinoma.</p><p> </p>

Highlights

Cervical cancer rate has shown increasing trend in the women of young age and comprises the second frequently detected cancer in females throughout the globe (Phongsavan et al, 2010; Wang et al, 2010)
The results from CCK-8 assay demonstrated that cell viability decreased to 24 and 29% in Bu25TK and HeLa cells, respectively compared to 100% in the control with the increase in concentration of plumericin from 10 to 40 μM (Figure 2)
Treatment of Bu25TK and HeLa cells with 40 μM concentration of plumericin for 12, 24 and 48 hours reduced the viability of Bu25TK cells to 86, 52 and 24%, respectively

Summary

Introduction

Cervical cancer rate has shown increasing trend in the women of young age and comprises the second frequently detected cancer in females throughout the globe (Phongsavan et al, 2010; Wang et al, 2010). It is estimated that every year around 275,000 deaths occur due to cervical cancer and half million cases are detected (Agarwal et al, 2011). The mechanisms underlying the cervical cancer is yet to be fully understood (Dursun et al, 2008). The early stages of the cervical cancer are asymptomatic which results in its detection at the advanced stage (Dursun et al, 2008). At present women are advised Pap tests at regular intervals which has increased the rate of cervical cancer detection at early stage making the treatment favorable (Parkin et al, 2005; Morice and Castaigne, 2005)

Methods

Results

Conclusion