Abstract
Icariin, the main effective component extracted from epimedium, has been shown to stimulate osteogenic differentiation and bone formation and to increase synthesis of the cartilage extracellular matrix. However, there has been little study on the effects of icariin on osteoarthritis. In this study, we loaded icariin onto poly(lactic-co-glycolic acid) (PLGA) electrospinning. The aim of this study was to explore a composite scaffold and to inhibit the progression of osteoarthritis. Our main experimental results demonstrated that the PLGA/icariin composite spinning scaffold had higher hydrophilicity, and icariin was released slowly and steadily from the scaffold. According to the results of an MTT test, immunofluorescence staining, an alkaline phosphate activating assay and a real-time polymerase chain reaction (RT-PCR) assay, the PLGA/icariin composite scaffold had good biocompatibility. In models of osteoarthritis, the results of a RT-PCR assay indicated that the PLGA/icariin scaffold promoted the synthesis of the extracellular matrix. The results of X-ray microtomography and histological evaluation demonstrated that the PLGA/icariin scaffold maintained the functional morphology of articular cartilage and inhibited the resorption of subchondral bone trabeculae. These findings indicated that the PLGA and icariin composite scaffold has therapeutic potential for use in the treatment of osteoarthritis.
Highlights
Osteoarthritis (OA) is the most common musculoskeletal disease, and it is generally characterized by cartilage degradation, subchondral& 2019 The Authors
Based on the effect of icariin on bone formation and cartilage matrix formation, we hypothesize that icariin can maintain the functional morphology of articular cartilage and the function of subchondral bone, which in turn prevents the progression of OA
The results indicated that poly(lactic-co-glycolic acid) (PLGA) or PLGA/icariin scaffolds could not induce the expression of AGG and collagen type I (Col-I) in mesenchymal stem cells
Summary
Osteoarthritis (OA) is the most common musculoskeletal disease, and it is generally characterized by cartilage degradation, subchondral& 2019 The Authors. Osteoarthritis (OA) is the most common musculoskeletal disease, and it is generally characterized by cartilage degradation, subchondral. The breakdown of cartilage, subchondral bone and other tissues 2 in the joints causes pain and the loss of range of motion [2]. The treatments for OA are based on three critical targets: relieve pain, improve joint function and delay the progression of the disease [3]. There are few studies on the effects of icariin on osteoarthritis. Based on the effect of icariin on bone formation and cartilage matrix formation, we hypothesize that icariin can maintain the functional morphology of articular cartilage and the function of subchondral bone, which in turn prevents the progression of OA
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