Year-end Sale % OFF 
Abstract
We undertook this study to investigate the influence of PLGA-Dermatophagoides protein 1 (Der p1) vaccine on the growth of Lewis lung cancer (LLC) cells in C57/BL/6 mice. After subcutaneous transplantation of LLC, cells were injected into the axilla of C57/BL/6 mice. Five mice received intraperitoneal (i.p.) injection of PLGA-Der p1 vaccine, and another five mice received i.p. injection of PBS or PLGA as control. Body weight of C57/BL/6 mice and tumor growth were measured. Morphological change of tumor was observed under optical microscope. The expression of Ki67 and CD34 protein was detected by immunohistochemistry analysis. The concentration of IL-4 and IFN-γ in the splenocyte culture medium was detected by ELISA. The growth of transplanted tumor was inhibited markedly by PLGA-Der p1 vaccine. The protein level of Ki67 and CD34 was significantly lower in PLGA-Der p1 vaccine group than in the control group (p<0.05). The level of IFN-γ of the splenocyte culture medium was significantly higher in PLGA-Der p1 vaccine group than that in the control group (p<0.05). However, the level of IL-4 in the splenocyte culture medium was obviously lower in PLGA-Der p1 vaccine group than that in the control group (p<0.05). PLGA-Der p1 vaccine has a significant inhibitory effect on the growth of LLC cells in C57/BL/6 mice by inducing the production of a Th1 cytokine profile.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
